Supplementary Figure 4: Reversal potential (Erev) of gain-of-function mutations affecting the hHCN1 channel.
From: De novo mutations in HCN1 cause early infantile epileptic encephalopathy
(a) Representative whole-cell current trace recorded from a CHO-K1 cell transfected with construct for wild-type (WT) hHCN1 channel. For determination of Erev, currents were fully activated by a prepulse to –130 mV for 1 s from a holding potential of –20 mV, and test pulses were then applied from –100 mV to +40 mV in 10-mV increments, with tail current measured immediately after the prepulse. (b) Plot of mean tail current density as a function of test voltage for WT, S100F, H279Y and D401H hHCN1 channels. The number of cells for each condition is indicated in parentheses. The red line represents a linear regression through the mean values for the tail current density and is used to obtain Erev. (c) Bar graph of Erev for WT hHCN1 and the three mutant channels (one-way ANONA followed by a Dunnett's test, ***P < 0.001). Data are represented as mean values ± s.e.m. with numbers of experiments given in parentheses.
