Supplementary Figure 5: Data analysis of loss-of-function mutations affecting the hHCN1 channel identified with the patch-clamp technique.
From: De novo mutations in HCN1 cause early infantile epileptic encephalopathy
Plot of mean current density as a function of test voltage for control cells (cells transfected only with pEGFP plasmid, reflecting the endogenous current) and cells expressing S272P and R297T mutant channels. The number of cells for each condition is indicated in parentheses. Currents were elicited by test pulses ranging from –140 mV to +40 mV in 10-mV increments from a holding potential of –20 mV and were normalized to cell capacitance (representative traces in Fig. 2a). Current densities of both mutant channels were not significantly different compared with endogenous currents from control cells (one-way ANOVA followed by a Dunnett's test, P > 0.05). Data are presented as means ± s.e.m. with numbers of experiments given in parentheses.
