Supplementary Figure 6: Gene set enrichment analysis on the gene list from MYOD1 L122R–transfected C2C12 cells. | Nature Genetics

Supplementary Figure 6: Gene set enrichment analysis on the gene list from MYOD1 L122R–transfected C2C12 cells.

From: A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations

Supplementary Figure 6

(a) GSEA plots for the top seven transcription factor binding motifs enriched among genes changed by the transfection of MYOD1 L122R in C2C12 cells. The genes from the microarray analysis are ranked according to their differential expression scores in MYOD1 L122R–transfected C2C12 cells from highest to lowest along the x axis. The over-representation of genes encoding DNA-binding motifs (represented by the black lines) at the top of the ranked gene list suggests that there is a correlation between genes encoding this binding motif and MYOD1 L122R–regulated genes. The green line represents the running enrichment score. (b) Table of the significant (FDR q value < 0.1) gene sets associated with the MYOD1 L122R–regulated gene signature. Sets are ranked in order of their normalized enrichment score (NES). The transcription factors associated with these DNA-binding motifs are shown.

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