Supplementary Figure 3: Introduction of MYOD1 L122R and PIK3CA H1047R into C2C12 mouse myoblast cells. | Nature Genetics

Supplementary Figure 3: Introduction of MYOD1 L122R and PIK3CA H1047R into C2C12 mouse myoblast cells.

From: A recurrent neomorphic mutation in MYOD1 defines a clinically aggressive subset of embryonal rhabdomyosarcoma associated with PI3K-AKT pathway mutations

Supplementary Figure 3

(a) Protein blot analysis of MYOD1 and MYC transfectants. The expression levels of MYOD1 (top panel), MYC (second from the top panel) and PI3 kinase p110α (third from the top panel) were evaluated. GAPDH is shown as a loading control (bottom panel). This confirmed MYOD1 protein expression and MYC protein expression in the respective transfectants. (b) Phase-contrast microscopy of C2C12 mouse cells transfected with GFP, wild-type MYOD1, MYOD1 L122R or MYC expression constructs, cultured in normal medium (not differentiation medium) (top panel; 200×magnification; scale bar, 100 μm). (c) Phase-contrast microscopy of C2C12 cells transfected with the indicated expression plasmids cultured in normal medium (not differentiation medium) are shown (200× magnification; scale bar, 100 μm). Arrowheads indicate multinucleated myotubes.

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