Supplementary Figure 2: Deletion series to delineate the MNE region.
From: Long-range enhancers regulating Myc expression are required for normal facial morphogenesis
(a) Schematic representation of the different deletions (red bars) generated and analyzed along the interval, with the different regulatory regions identified (blue, medionasal enhancer (MNE); orange, nasal epithelial enhancer (NEE)) shown as ovals. (b–e) LacZ staining of E11.5 embryos with different deletions, highlighting the persistence or loss of the two expression domains (blue arrowhead, MNP; orange arrowhead, NC). Insets in c–e, 150-μm vibratome sections through the head of embryos, showing strong expression in the nasal epithelium of del(8–14) heterozygous embryos (c). This domain of staining is absent in del(14–15) embryos (d) and weak but present in del(15–17) embryos (e).
