Supplementary Figure 4: Representative qPCR and immunohistochemistry studies confirm differential gene expression in SNS.

(a,b) High expression of NTRK3 and MYOD1 was observed in most examples of SNS. (c) MLANA and MITF (data not shown) were not expressed in SNS. (d) NTRK3 showed a strong membranous pattern of expression in virtually all SNS samples (24/25). No staining was observed in the sinonasal epithelium. (e) MYOD1 nuclear expression was focal in a few SNS tumors (4/25); the arrow indicates an example cell with focal MYOD1 expression. (f) Melan A and MITF (data not shown) expression was universally negative in all SNS tumors tested (n = 22). qPCR and/or immunohistochemistry also confirmed the overexpression of PLUNC, PCP4, GDF7, PIK3C2G, ALX1 and PAX3 and the downregulation of HOXB7 (data not shown; see also Supplementary Table 1; additional information about the SNS phenotype can be found in ref. 1). RE, relative expression; SNS, sinonasal sarcoma (n = 5); NST, normal sinonasal tissue (n = 4); ARMS, alveolar rhabdomyosarcoma (n = 3); DFSP, dermatofibrosarcoma protuberans (n = 3); MPNST, malignant peripheral nerve sheath tumor (n = 3); SS, synovial sarcoma (n = 3). *** and ** indicate Kruskal-Wallis test P < 0.01 and P < 0.05, respectively, and post-hoc Dunett's test P < 0.05; * indicates Wilcoxon-Mann-Whitney test P < 0.05; data are shown as means ± s.d.