Supplementary Figure 5: Differential expression of myogenin in SNS and ARMS.

(a) Biphenotypic SNS is a monomorphic spindle cell neoplasm that exclusively occurs in the head and neck region of middle-aged individuals, most commonly in females (hematoxylin and eosin, ×100). (b) ARMS is a pediatric small round cell tumor that shows a characteristic pseudoalveolar growth pattern (hematoxylin and eosin, ×100). (c,d) qPCR and immunohistochemical analyses show that, whereas all ARMS tumors (n = 11) express high levels of MYOG (myogenin) mRNA (c) and protein (d, inset), SNS tumors (n = 11) are consistently negative for this marker (c,d). *P < 0.0001, Wilcoxon-Mann-Whitney test; data are shown as means ± s.d.