Supplementary Figure 4: C2CD3 localizes to centrioles and procentrioles.
From: The oral-facial-digital syndrome gene C2CD3 encodes a positive regulator of centriole elongation
(a,b) IMCD3-[GFP-C2CD3] cells stained for (a) centrin (red) and glutamylated tubulin (GT335; pink). Centrin marks the centrioles and procentrioles where C2CD3 is also found. Meanwhile, glutamylated tubulin is only found at the mature centrioles. Importantly, C2CD3 appears to mark a distal structure of the centriole coinciding with centrin, while glutamylated tubulin marks the entire centriole and generates a diffraction-limited spot at the center of the centriole. (b) ODF2 (red; marks the subdistal appendages) and glutamylated tubulin (GT335; blue). All cells were treated with nocodazole before processing for immunofluorescence. All scale bars are 1 μm. (c) GST capture assays, supplement to Figure 2j. The same samples as in Figure 2j were resolved by SDS-PAGE and stained with Coomassie to assess the amounts of the GST-OFD1 fusion pulled down by glutathione-sepharose beads. Asterisks denote full-length GST-OFD1 truncations.
