Supplementary Figure 7: Characterization of the C2CD3-induced elongated structures.
From: The oral-facial-digital syndrome gene C2CD3 encodes a positive regulator of centriole elongation
(a) U2OS cells transfected with GFP-C2CD3 were treated with vehicle (top) or 5 μM nocodazole (bottom) for 1 h followed by fixation and staining for tubulin (DM1A; white), acetylated tubulin (red) and DNA. The diffuse tubulin staining in nocodazole-treated cells corresponds to the free tubulin dimers in the cytoplasm. Scale bars, 5 μm. (b) Serial sections of the hyperelongated centriole shown in Figure 5f. (c) RPE cells transfected with GFP-C2CD3 were stained for tubulin (red). GFP-C2CD3 colocalizes extensively with bundles of microtubules. Scale bar, 5 μm.
