Supplementary Figure 10: MALDI-TOF mass spectrometry of permethylated O-glycans from healthy and JAGN1-mutated human peripheral blood and bone marrow neutrophil samples.
From: JAGN1 deficiency causes aberrant myeloid cell homeostasis and congenital neutropenia
MALDI-TOF mass spectrometry of permethylated Ο-glycans from (a) the healthy mother from pedigree C, (b) the healthy father from pedigree C, (c) an unrelated healthy donor, (d) an unrelated healthy donor additionally stimulated with G-CSF, (e) patient P8, (f) patient P7, (g) patient P12 and (h) patient P3. Peripheral blood neutrophil granulocytes were available for a–g; for patient P3, neutrophil granulocytes from bone marrow were assessed. O-glycomic profiles of permethylated O-glycans are from the 35% MeCN fraction (Online Methods). Putative structures are based on composition, tandem mass spectrometry and biosynthetic knowledge.
