Supplementary Figure 14: Immunofluorescence-based localization studies using GFP-tagged JAGN1 constructs.
From: JAGN1 deficiency causes aberrant myeloid cell homeostasis and congenital neutropenia
HeLa cells (a,b) and fibroblasts (c,d) transiently transfected with constructs expressing GFP or fusion proteins (N-terminally fused EGFP-JAGN1 or C-terminally fused JAGN1-EGFP) were counterstained using an antibody either against the ER marker protein calnexin or the Golgi apparatus marker protein golgin-97. Live-cell staining was performed with MitoTracker for mitochondria staining. In all cases, the characteristic reticulated staining pattern of the ER is observed. The far right panel depicts the merged images for GFP (green) and the specific cell organelle (red), where colocalization of the signals is demonstrated in yellow. The overlay shows that colocalization of calnexin and JAGN1 is extensive but incomplete. There is no colocalization of JAGN1 and the Golgi apparatus or mitochondria.
