Supplementary Figure 4: Western blot showing specificity of the ABCA1 antibody.
From: Common variants near ABCA1, AFAP1 and GMDS confer risk of primary open-angle glaucoma
Protein lysates of NTM-5 (NTM) and GTM-3 (GTM) human trabecular meshwork cells, respectively, from a normal control and an individual with glaucoma were analyzed by SDS-PAGE and western blotting performed using the mouse anti-ABCA1 antibody. The >250 kDa protein band detected in both the cell types is greater than the expected size of ABCA1 (254 kDa) and corresponds to the post-translationally modified form of the protein. The ∼70 kDa band detected in both the samples has been reported previously but its identity remains unknown. The blot was overexposed to improve visibility of the expected protein species. Red indicates oversaturation of signal. The minor protein bands seen in both the cell types are likely degradation products of the full-length protein and were not visible in a shorter exposure. kDa = kilodaltons.
