Supplementary Figure 5: Distribution of the AFAP1 protein in human ocular tissues.
From: Common variants near ABCA1, AFAP1 and GMDS confer risk of primary open-angle glaucoma
(a-f) Sections of a normal human eye were immunolabelled with the anti-AFAP1 antibody (brown) (see Supplementary Figure 6 for antibody specificity) and counterstained with hemotoxylin to visualize nuclei (blue). Positive immunolabelling was detected in the trabecular meshwork (a,b), retina (c,d) and optic nerve (e,f). In the retina (c,d), AFAP1 immunolabelling was prominent in the photoreceptor inner segment (IS), in some cells in the inner nuclear layer (INL), in retinal ganglion cells in the ganglion cell layer (GCL) and retinal blood vessel wall (not shown). In the optic nerve (e,f), AFAP1 protein was primarily present in the astrocytes. (g,h) In sections of a glaucomatous eye, similar AFAP1 distribution was observed (data not shown). Labelling in the layers of the retina (g,h) was also similar to that in a normal eye. Positive labelling in the blood vessel wall is marked with an arrow. The experiment was repeated for reproducibility. sc, Schlemm’s canal; IPL, inner plexiform layer; OPL, outer plexiform layer; ONL, outer nuclear layer; OS, outer segment; RPE, retinal pigment epithelium. Scale bar = 100 μm (a-d, f-g); 500 μm (e).
