Supplementary Figure 8: NLRC4-MAS and NOMID macrophages have comparable production of TNF-α and IL-10.
Monocytes and monocyte-derived macrophages were isolated from healthy controls, the NLRC4-MAS patient and an NOMID patient (Online Methods). Cells were stimulated as indicated, and secreted cytokines were measured by Luminex. (a) NLRC4-MAS monocytes from before and after treatment with IL-1 receptor antagonist were stimulated, and secreted cytokines were measured by Luminex. (b) Pre- and post-treatment monocyte-derived macrophages were stimulated, and secreted cytokines were measured by Luminex. Columns represent the mean and s.d. of technical duplicates. No appreciable secretion of IL-12p70 or IFNα2 was detected for any sample (data not shown). FLA-hi, flagellin (5 μg/ml); IC-FLA lo, liposomal flagellin for intracellular delivery (1 μg/ml); IC-FLA-hi (5 μg/ml).
