Supplementary Figure 9: T337S NLRC4–transduced THP1 cells have a proliferative disadvantage. | Nature Genetics

Supplementary Figure 9: T337S NLRC4–transduced THP1 cells have a proliferative disadvantage.

From: An activating NLRC4 inflammasome mutation causes autoinflammation with recurrent macrophage activation syndrome

Supplementary Figure 9

THP1 cells were transduced with an empty vector, mutant NLRC4 or various dilutions of wild-type NLRC4 retrovirus. Equal amounts of plasmid construct were used to generate virus-containing supernatant. Mutant and wild-type constructs differed by 1 bp. Transduction efficiency was assessed at the indicated time points by flow cytometry for GFP expression. Median fluorescence intensities for the GFP-positive population are depicted.

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