Supplementary Figure 9: Yeast one-hybrid analysis indicates that BvMYB1 can bind to upstream regulatory regions of BvDODA1 and BvCYP76AD1.

Sequence upstream of the translation start codons for two betalain structural genes, BvDODA1 and BvCYP76AD1, and a putative phenylpropanoid gene, BvDFR, were fused upstream of the HIS3 reporter gene. The upper panels show that these upstream regions do not activate the reporter in the absence of BvMYB1 expression. The lower panels show that BvMYB1 is able to activate BvDODA1 and BvCYP76AD1 but not BvDFR.