Supplementary Figure 5: Characterization of SB-Smad4 and SB-Rspo2 fusion transcripts. | Nature Genetics

Supplementary Figure 5: Characterization of SB-Smad4 and SB-Rspo2 fusion transcripts.

From: Transposon mutagenesis identifies genes and evolutionary forces driving gastrointestinal tract tumor progression

Supplementary Figure 5

(a) A chromatogram showing the partial sequence of an SB-Smad4 fusion tumor transcript. The transcript contains Smad4 exon2, which was replaced with the PGK-neo expression cassette in the knockout allele. This shows that the transposon is inserted in the wild-type Smad4 allele. (b) Primer design used to amplify by RT-PCR SB-Rspo2 fusion tumor transcripts. Black arrows denote primers. (c) A chromatogram showing a partial sequence of the SB-Rspo2 fusion transcript. Note that the transposon splice donor (SD) site is spliced to Rspo2 exon 2, which contains the ATG initiation codon. Full-length Rspo2 protein is thus overexpressed following SB insertion upstream of Rspo2. ex, exon.

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