Supplementary Figure 4: Cell fractionation shows decreased nuclear localization of mutant ETV6.
From: Germline ETV6 mutations in familial thrombocytopenia and hematologic malignancy
HeLa cells were transfected with empty vector, cDNA for wild-type ETV6 or ETV6 cDNA encoding p.Arg399Cys. Lysates of nuclear versus cell fractions as well as whole-cell lysates were analyzed by western blot for ETV6, GAPDH (cytoplasmic marker) and NPM1 (nuclear marker).
