Supplementary Figure 4: Expression of CEL protein variants in vitro and in acinar cells. | Nature Genetics

Supplementary Figure 4: Expression of CEL protein variants in vitro and in acinar cells.

From: A recombined allele of the lipase gene CEL and its pseudogene CELP confers susceptibility to chronic pancreatitis

Supplementary Figure 4

(a) In vitro transcription and translation of the CEL-WT and CEL-HYB expression vectors employed in cellular studies. Both protein variants exhibited their predicted unglycosylated molecular sizes (100 kDa and 65 kDa, respectively) when detected by western blotting. (b) Expression of CEL-WT and CEL-HYB in acinar cells. Mouse 266-6 cells were transiently transfected with the CEL-WT or CEL-HYB expression vectors for 24 hours, and cell lysates (L) and media (M) were analyzed by western blotting. Transfection of empty vector (EV) was included as negative control. The actin bands indicate the amount of loaded protein in the lysate lanes only. In both a and b, the blots are representative of three independent experiments.

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