Supplementary Figure 5: Representative example of results from the LightCycler assay used for primary CEL-HYB screening.

The image shows the melting curves of 96 samples, including six no-template controls. The melting point of the amplified CEL-HYB allele is about 8 °C higher than for the CEL-WT (58 °C vs. 50 °C). Three samples were scored as CEL-HYB-positive and 87 as negative.