Supplementary Figure 7: SNX14 is involved in autophagic regulation.
(a) Cell fractionation from human NPCs grown under starvation conditions to induce autophagy. SNX14 is enriched in the compartment with the strongest signal for LC3-II (lane 4) and in endosomal/lysosomal compartments (red). (b) SNX14 dynamically localizes to vesicles positive for LC3 upon induction of autophagy (2-h treatment with EBSS) in wild-type human NPCs expressing dsRED-SNX14. (c) Immunoblot analysis of LAMP1, BECLIN1, cathepsin D and p62, with quantification by densitometry. α-tubulin was used as a loading control. The graph presents means ± s.d. (n = 3 clones). *P < 0.05 (two-tailed t test).
