Supplementary Figure 2: Mycobacterium tuberculosis Rv3711c (Rv3711cMTB) is a 3′–5′ DNA exonuclease but does not form a stable complex with DnaE1MTB. | Nature Genetics

Supplementary Figure 2: Mycobacterium tuberculosis Rv3711c (Rv3711cMTB) is a 3′–5′ DNA exonuclease but does not form a stable complex with DnaE1MTB.

From: DNA replication fidelity in Mycobacterium tuberculosis is mediated by an ancestral prokaryotic proofreader

Supplementary Figure 2

(a) Coomassie-stained gel showing purified E. coli ɛ (ɛEC), DnaE1MTB and Rv3711cMTB. (b) Gel showing a 3′–5′ exonuclease activity assay with ɛEC, DnaE1MTB and Rv3711cMTB. (c) Analytical size exclusion chromatography shows that E. coli PolIIIα (PolIIIαEC) and ɛEC form a stable complex at concentrations as low as 1.5 μM. (d) In contrast, DnaE1MTB and Rv3711cMTB do not show any interaction, even at 10 μM protein concentration (all equimolar amounts).

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