Supplementary Figure 5: IC50 measurement of cGMP.
From: PDE3A mutations cause autosomal dominant hypertension with brachydactyly
cGMP competitively inhibits PDE3A, but nearly equivalent values were determined for Flag-tagged wild-type (WT) PDE3A1 and the tagged PDE3A1-T445N mutant. IC50 was 5.164 µM for WT and 4.78 µM for T445N (n = 3). Relevant differences were not found in three independent experiments.
