Supplementary Figure 8: PDE3A overexpression in HeLa cells and carboxyfluorescein diacetate succinimidyl ester (CFSE) signal quantification in FACS analysis.
From: PDE3A mutations cause autosomal dominant hypertension with brachydactyly
Intensity histogram of flow cytometry analysis of CFSE-labeled and transfected HeLa cells. After CFSE labeling, the cells were seeded and were transfected the next day with full-length wild-type PDE3A or full-length PDE3A expressing the six mutations. Measurements were performed at 24, 48 and 72 h after transfection to determine the proliferation rates. Because of mitosis and the distribution of CFSE, the signal intensity changed in the measured time of 72 h.
