Supplementary Figure 12: Expression of PTHrP and VASP in MSCs and VSMCs.
From: PDE3A mutations cause autosomal dominant hypertension with brachydactyly
The color spectrum indicates expression levels from low (black) to high (red) in semiquantitative immunofluorescence. (a) PTHrP influences the proliferation of VSMCs (Song, G.J., Fiaschi-Taesch, N. & Bisello, A. Mol. Endocrinol.23, 1681-1680, 2009). Full-length PTHrP protein levels were increased, whereas PTHrP peptide levels were decreased, in MSCs and VSMCs from the patient harboring the T445N substitution. (b) Phosphorylation of VASP at Ser157 was reduced in VSMCs from the affected patients compared to controls; phosphorylated VASP was not detectable in MSCs.
