Supplementary Figure 13: Peptide assays for the PDE3A mutants.
From: PDE3A mutations cause autosomal dominant hypertension with brachydactyly
(a) Amino acid sequences of the synthesized peptide spots from Figure 5a. Serine residues (S; red) at position 428 or 438 were replaced by alanine (A; blue) or aspartic acid (D; green) or with prephosphorylated serine (pS; pink). Positions 445, 447 and 449 for the altered amino acids are marked in yellow; the altered amino acid is marked in gray. (b) Phosphorylation of two peptide spot membranes (30-mers representing PDE3A Ile421-Leu450; see Fig. 2a) without (– PKA) and with (+ PKA) PKA. The peptide spots for PDE3A-WT and PDE3A-T445N (black frame) are the same signals, shown in Figure 5a. (c) Amino acid sequences of the synthesized peptide spots from b. The color code is as indicated above. Serine residues at position 428 or 438 were replaced by alanine or aspartic acid or by prephosphorylated serine. Positions 445, 447 and 449 corresponding to the altered amino acids are shown in yellow; each altered amino acid is marked in gray.
