Supplementary Figure 1: Generation of Trbp knockout mice.
From: Trbp regulates heart function through microRNA-mediated Sox6 repression
(a) Schematic of the Trbp knockout strategy. (b) Southern blot and PCR assays to confirm the desired recombination events. (c) qRT-PCR of Trbp expression in the indicated organs of Trbp-null (Trbp−/−) and wild-type (WT) mice. n = 3; **P < 0.01. (d) Left, Kaplan-Meier survival curves of Trbp−/− and WT mice. Middle, gross morphology of 2-week-old Trbp−/− and WT mice. Right, body weight of 2-week-old Trbp−/− and WT (+/+) mice. n = 6; **P < 0.01.
