Supplementary Figure 3: Principal-component analysis of mutational context.

The counts of each different mutational context in each of the three sets of SNVs (common, Barrett's esophagus (BE) unique, EAC unique) were used as input for a principal-component analysis. There is some separation between the common SNVs and the unique SNVs in the second component, but the differences between the groups are not striking (6.9% variance in the second component). (b) Plot showing the weights of the 96 different mutational contexts. Four mutational contexts—C(A>C)C, G(C>G)G and C(A>T)C (all at the top right-hand side of the plot) as well as T(C>G)A (bottom right)—seem to be the main cause for the separation in the second component.