Supplementary Figure 5: EGR2 is a downstream component of the FGF pathway in Ewing sarcoma.
From: Chimeric EWSR1-FLI1 regulates the Ewing sarcoma susceptibility gene EGR2 via a GGAA microsatellite
(a) Scatter-dot plot and medians (horizontal bars) of EGFRs (gray) and FGFRs (red) expression levels in n = 117 primary Ewing sarcoma tumors (GSE34620). (b) Analysis of proliferation of Ewing sarcoma cell lines with a Resazurin assay. Cells were seeded in RPMI 1640 medium with 0.5–1% FCS and stimulated with the indicated growth factor (GF) concentrations for 72 h. Data are shown as the mean and s.e.m.; n ≥ 6 experiments. (c) Analysis of EGR2 induction by qRT-PCR in Ewing sarcoma cell lines after incubation with either EGF or bFGF (both 10 ng/ml). The EGFR expressing MDA-MB-231 breast cancer cell line served as a positive control for EGF action. Data are shown as the mean and s.e.m.; n ≥ 3 independent experiments. Two-tailed unpaired Student’s t-test, ** P < 0.01, *** P < 0.001.
