Supplementary Figure 8: Sequence confirmation of CRISPR-induced lesions in Mmp21.
From: MMP21 is mutated in human heterotaxy and is required for normal left-right asymmetry in vertebrates
Chromatograms showing Mmp21 mutations found in yolk sac DNA or in cloned PCR products amplified from yolk sac DNA, from F0 CRISPR-targeted mouse embryos. (a) Mutant embryo I226T-7 exhibits a homozygous 7-bp deletion, and embryo I226T-1 is compound heterozygous for a deletion in one allele (4/11 bacterial clones) and a knock-in of the desired A-to-G mutation causing the p.Ile226Thr substitution (in conjunction with three other synonymous single-base mutations; only two are shown here) on the other allele (7/11 bacterial colonies). (b) The A321P-12 mutant embryo has biallelic deletions predicted to cause frameshifts. Guides used in these experiments are shown in blue with the PAM sequence in green, and the corresponding orthologous human mutation site is shown in yellow.
