Supplementary Figure 7: Induction of aberrant splicing by a splice-blocking mmp21 morpholino.
From: MMP21 is mutated in human heterotaxy and is required for normal left-right asymmetry in vertebrates
(a) Schematic of the mmp21 zebrafish gene showing the location of MO1 targeting the exon 4/intron 4 splice donor and MO2 targeting the intron 4/exon 5 acceptor. 5′ and 3′ ISH probes are indicated by violet bars. (b) Electrophoresis gel after PCR performed on cDNA (using primers P3 and P6) from wild-type (wt) embryos and from embryos injected with MO1 or MO2. PCR product from primers P3/P6 runs at 584 bp in wild-type embryos. MO1 causes aberrant splicing, indicated by the black asterisk (new aberrant splice product). The red asterisk indicates that wild-type transcript levels are reduced by approximately 75% by MO1. MO2 does not cause aberrant splicing (white asterisk).
