Supplementary Figure 7: Assessment of cardiac regurgitation in zebrafish morpholino knockdown for genes on Chr17p13.
From: Genetic association analyses highlight biological pathways underlying mitral valve prolapse
(a) Genomic context of the association signal observed in the GWAS meta-analysis. (b) Morpholino-mediated knockdown efficacy. Efficacy for smg6 and sgsm2 in embryonic zebrafish was measured by RT-PCR. (c) Fold change in observed mitral regurgitation in 72-hpf zebrafish embryos after morpholino-mediated knockdown. All results are relative to clutchmate controls. n = number of biological replicates per morpholino. (d) Brightfield micrographs displaying gross morphology of 72-hpf embryos following smg6 or sgsm2 knockdown. Scale bar represents 1 mm. CN = control morpholino-injected embryos.
