Supplementary Figure 10: Electrophoretic mobility shift assay of rs10830963 in HepG2 cellular extracts. | Nature Genetics

Supplementary Figure 10: Electrophoretic mobility shift assay of rs10830963 in HepG2 cellular extracts.

From: Genetic fine mapping and genomic annotation defines causal mechanisms at type 2 diabetes susceptibility loci

Supplementary Figure 10

Nuclear extract (NE) from HepG2 cells was treated with labeled probes of 25 bp of sequence containing each allele of rs10830963. We observed protein bands bound to both sequences (black) as well as bands unique to the non-risk (red) and risk (blue) sequences. We then introduced antibodies against HNF3B (FOXA2 alias) and four factors (YY1, TAL1, NEURDO1 and PTF1A) whose known binding motifs match the de novo motif at rs10830963. We observed no shifting of the allele-specific bands with any antibody. Several bands shared by both alleles were removed in the presence of antibody to YY1.

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