Table 1 Segregation ratios demonstrating apparent lethality of inversion genotypes in matings between heterozygotes identified with diagnostic SNPs

From: A supergene determines highly divergent male reproductive morphs in the ruff

Phenotype associated with SNP

SNPa

Progeny

 

Genotype

Deviation from 1:2:1

Deviation from 1:2b

 

+/+

+/I

I/I

χ 2 2

P

χ 2 1

P

Faeder

A and B

All

Obs

20

16

0

22.67

1.2 × 10−5

8.00

0.005

   

Exp

9

18

9

    
  

Males

Obs

8

7

0

8.60

0.014

2.70

0.100

   

Exp

3.75

7.5

3.75

    
  

Females

Obs

12

9

0

14.14

8.5 × 10−4

5.36

0.021

   

Exp

5.25

10.5

5.25

    

Satellite

C

All

Obs

20

18

0

21.16

2.5 × 10−5

6.37

0.012

   

Exp

9.5

19

9.5

    
  

Males

Obs

9

9

0

9.00

0.011

2.25

0.134

   

Exp

4.5

9

4.5

    
  

Females

Obs

11

9

0

12.30

0.002

4.23

0.040

   

Exp

5

10

5

    
  1. Results were obtained by pooling the data for all offspring across reproductive events where both parents were heterozygous for diagnostic SNPs. I, the inversion haplotype (associated with either Faeder or Satellite); +, the ancestrally ordered haplotype (associated with independent); obs, observed; exp, expected; χ2, goodness-of-fit chi-squared with degrees of freedom.
  2. aSNPs used: A, contig 1270: 576,631; B, contig 3047: 368,535; C, contig 1270: 576,631. The sex ratios produced by these crosses show no suggestion of sex-specific lethality, that is, no sex-dependent difference in the +/+:+/I ratio (SNPs A, B and C, Fisher's exact test, P = 1.00).
  3. bExcluding the inversion homozygote (I/I) class.
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