Supplementary Figure 11: NUDT15 genotype and its protein stability in vitro.
From: NUDT15 polymorphisms alter thiopurine metabolism and hematopoietic toxicity
Variant or wild-type NUDT15 cDNA encoding an N-terminal FLAG tag was transiently expressed in HEK293T cells using polyethylenimine reagent. Cycloheximide (CHX; 50 mg/ml) was added 48 h after transfection, and NUDT15 protein level was monitored after 0, 24 and 48 h by immunoblotting with β-actin as the loading control. Immunoblot images are shown (top), and protein level was quantified using ImageJ software (bottom).
