Supplementary Figure 12: Expression and purification of wild-type and variant human NUDT15 proteins.
From: NUDT15 polymorphisms alter thiopurine metabolism and hematopoietic toxicity
N-terminally His-tagged human NUDT15 was expressed in E. coli BL21 with IPTG induction and purified by affinity chromatography. One microgram of protein was loaded onto a 4–15% SDS-PAGE gel for electrophoresis, and the gel was stained with Coomassie Brilliant Blue.
