Supplementary Figure 2: Enzymatic activity of wild-type and variant NUDT15 with TdGTP as the substrate. | Nature Genetics

Supplementary Figure 2: Enzymatic activity of wild-type and variant NUDT15 with TdGTP as the substrate.

From: NUDT15 polymorphisms alter thiopurine metabolism and hematopoietic toxicity

Supplementary Figure 2

(a) Each variant NUDT15 was expressed in E. coli, and purified protein was subjected to diphosphatase activity measurement with TdGTP as the substrate. (b) Variant or wild-type proteins were combined to determine the level of NUDT15 activity in patients with different diplotypes. Each experiment was performed in triplicate and was repeated at least three times. Center values (dots) represent the means of triplicate experiments; error bars, s.d.

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