Supplementary Figure 3: Thermostability assay for wild-type and variant NUDT15.
From: NUDT15 polymorphisms alter thiopurine metabolism and hematopoietic toxicity
(a–f) Purified NUDT15 was incubated with SyproOrange, and the mixture was heated from 20 ºC to 95 ºC in increments of 0.2 ºC in the Quant Studio 12K Flex Real-Time PCR system. The temperature midpoint for protein unfolding transition, Tm (indicated by the red arrows), was calculated on the basis of the Boltzmann model. Experiments were performed in triplicate to estimate standard deviation.
