Supplementary Figure 10: Knockdown of NUP93 but not NUP188 decreases SMAD reporter activity upon BMP7 stimulation.
From: Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome
HEK293 cells were transfected with luciferase reporter constructs under the control of a SMAD-responsive promoter allowing measurement of SMAD activity in response to BMP7 stimulation (rc-BMP7). As compared to scrambled control siRNA, knockdown of NUP93 significantly reduces SMAD reporter activity (lane 2 versus 3). Knockdown of NUP153, encoding a nuclear basket protein, induces less pronounced but significant reduction of SMAD reporter activity. Knockdown of NUP188, encoding a direct interactor of NUP93, does not reduce SMAD reporter activity.
