Supplementary Figure 11: SMAD1/5 phosphorylation upon BMP7 treatment and NUP93 knockdown in HEK293 cells.
From: Mutations in nuclear pore genes NUP93, NUP205 and XPO5 cause steroid-resistant nephrotic syndrome
Treatment of HEK293 cells with recombinant BMP7 induces phosphorylation of SMAD1/5 after 60 min (top). Transfection of HEK293 cells with shRNA against human NUP93 results in efficient knockdown of NUP93 as compared to scrambled control (middle). The β-actin control (bottom) confirms equal loading.
