Supplementary Figure 7: In vivo requirement of motifs 4 and 5 for SBE1 activity.
From: Cis-regulatory architecture of a brain signaling center predates the origin of chordates
(a–c) X-gal staining of transgenic embryos expressing wild-type or mutant versions of the SBE1-lacZ construct at E10.5. The extent of zli staining is indicated by the length of the red bracket. Reduced zli staining was observed upon deletion of motif 4 or 5. The number of stained embryos out of the total carrying a given transgene is indicated. Scale bars, 1 mm. (d) Quantification of the spatial distribution of X-gal staining in the zli normalized to head size (*P < 0.01, **P < 0.001, Student’s t test).
