Supplementary Figure 4: RNA-seq profile of the SBE1 domain.
From: Cis-regulatory architecture of a brain signaling center predates the origin of chordates
(a) An E10.5 embryo from the 429M20eGFP BAC transgenic line showing GFP fluorescence in the SBE1 domain (dotted line), encompassing the ventral midbrain, ventroposterior diencephalon and zli. Reporter activity is also detected in the floor plate of the hindbrain and spinal cord. RNA was isolated from cells outlined by the dashed line for RNA-seq analysis. (b) A heat map of gene expression in the SBE1 domain showing FPKM values for transcriptional regulators of SBE1. (c–f) Whole-mount in situ hybridization for candidate transcription factors expressed in the SBE1 domain at E10.5. Scale bars, 1 mm.
