Supplementary Figure 4: Restoration of wild-type ald in BCG inhibits growth in the presence of higher concentrations of d-cycloserine. | Nature Genetics

Supplementary Figure 4: Restoration of wild-type ald in BCG inhibits growth in the presence of higher concentrations of d-cycloserine.

From: Genomic and functional analyses of Mycobacterium tuberculosis strains implicate ald in D-cycloserine resistance

Supplementary Figure 4

As BCG, which is innately resistant to d-cycloserine, contains a frameshift mutation in ald, a wild-type copy of M. tuberculosis ald was used for complementation back into BCG (BCG-ald-comp). Both BCG and BCG-ald-comp were subsequently cultured in varying concentrations of d-cycloserine in MGIT, and the time to positivity was normalized to that for the no-drug control for each strain to calculate the growth inhibition index. P values were calculated using two-way ANOVA.

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