Supplementary Figure 3: HEK293T expression of wild-type and Leu129Gln CysLT2R.
From: Recurrent activating mutations of G-protein-coupled receptor CYSLTR2 in uveal melanoma
(a) Immunoblotting for the N-terminal FLAG tag, C-terminal 1D4 tag, and -tubulin in HEK293T cells transfected with empty-vector, wild-type, or Leu129Gln CysLT2R. (b) Representative image of immunofluorescence for 1D4 (green) and FLAG (red) in HEK293T cells transfected with wild-type CysLT2R, or Leu129Gln CysLT2R. Hoechst (blue) was used to counterstain the nucleus. Scale bar, 10 m. (c) Flow cytometry analysis of live transfected HEK293T cells using FLAG antibody against the extracellular FLAG tag showing the respective cell surface expression of the receptor.
