Supplementary Figure 13: Analysis of the synthetic normal analog comparisons used for AML cell types.

(a) Pearson correlations derived from comparison of all peaks for AML cell types with the closest normal analog (color) and the enhancer cytometry–derived synthetic normal (gray). (b) Total significant peaks observed after comparison of AML cell types to synthetic normal analogs. Significant peaks were defined as those with a log₂ (fold change) value greater than 3 or less than –3 for comparison of AML with the closest normal or synthetic normal analog. (c) Comparison of AML cell types to synthetic normal analogs as shown in Figure 6i except reordered by patient instead of AML cell type. For each sample, the closest normal is shown with the color of the bar. The percentage of the total significant peaks that were removed by comparison to synthetic normal analogs is plotted for each sample. (d) Global epigenetic variance of AML cell types after subtraction of signal corresponding to synthetic normal analogs. Values are normalized to the maximum variance observed in Figure 5d to allow for comparison across plots.