Supplementary Figure 4: Multiple-sequence alignment of SNORD118 sequences. | Nature Genetics

Supplementary Figure 4: Multiple-sequence alignment of SNORD118 sequences.

From: Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts

Supplementary Figure 4

SNORD118 homologs were identified using Ensembl and Rfam and aligned using CLUSTALW. The alignment was manually refined using RALEE. Nucleotide alterations (not including deletions, insertions and duplications) identified in patients with LCC are annotated above the sequence. From 5′ to 3′, the C box, LSm-binding sites and D box are highlighted by red lines. Aligned columns are colored according to sequence conservation: black, >80% identity; dark gray, >60% identity; light gray, >40% identity. A possible base-paired secondary structure, based on the consensus U8 structure in the Rfam database and analysis with RNAalifold, is shown in dot-bracket notation below the alignment. The following sequences were used for alignment: Homo sapiens, ENST00000363593; Pan troglodytes, ENSPTRT00000052278; Macaca mulatta, ENSMMUT00000034372; Bos taurus, ENSBTAT00000060500; Canis familiaris, ENSCAFT00000034685; Mus musculus, ENSMUST00000082965; Oryctolagus cuniculus, ENSOCUT00000018667; Gallus gallus, ENSGALT00000043652; Xenopus tropicalis, ENSXETT00000065858; Tetraodon nigroviridis, ENSTNIT00000023953; Danio rerio, ENSDART00000115749.

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