Supplementary Figure 5: In silico analysis of variants in patients with LCC.

In silico analysis of variants located in the stem of a highly conserved hairpin loop from the predicted structure of U8. Bases 96–124 of the wild-type sequence of U8 are shown with patient variants underneath. The predicted base-paired secondary structure of each sequence is shown in dot-bracket notation, together with the minimum folding free energy (kcal/mol). Each of the three variants detected in patients with LCC is predicted to decrease the stability of the hairpin.