Supplementary Figure 7: U8 snoRNA precursor processing control experiments. | Nature Genetics

Supplementary Figure 7: U8 snoRNA precursor processing control experiments.

From: Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts

Supplementary Figure 7

(a) 3′-end precursor processing of precursor U8 variants in the absence of RNasin. Removal of commercially available RNase inhibitors (RNasin, Promega) does not alter the in vitro 3′-end processing of 5′-end-labeled precursor U8 snoRNA (U8-165) in HeLa nuclear extracts. (b) 3′-end precursor processing of 5′-end-labeled precursor U8 variant n.58A>G. At 30 min, the pattern of processing intermediates is the same as for wild-type RNA.

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