Supplementary Figure 13: TMEM107/TMEM107 expression (RNA/protein). | Nature Genetics

Supplementary Figure 13: TMEM107/TMEM107 expression (RNA/protein).

From: Mutations in SNORD118 cause the cerebral microangiopathy leukoencephalopathy with calcifications and cysts

Supplementary Figure 13

(a) qRT–PCR of TMEM107 expression in primary fibroblast cell lines from three controls and four patients with LCC, normalized to two housekeeping genes, HPRT1 and 18S. RQ is equal to 2− ΔΔCt, that is, the normalized fold change relative to a control. (b) Immunoblot of TMEM107 in primary fibroblast cell lines from three controls and five patients with LCC. Whole-cell lysates were derived from 5 × 106 cells per sample, and 30 μg of total protein was loaded per lane. Immunoblotting for actin (42 kDa) was used as a loading control.

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