Supplementary Figure 1: Histopathological characteristics of LCC.

(a,b) Cerebellar brain biopsy taken from F1172 at the age of 50 years showing numerous blood vessels, with groups of vessels that resemble angioma. (c,d) The vessels appear ectatic, partially thickened and hyalinized, and demonstrate an irregular vessel wall. Inflammatory changes are not evident. (d–f) Numerous macrophages with hemosiderin deposits around vessels (arrows) (d,e) and diffusely within the tissue (f) indicate old hemorrhage. (g,h) Both vascular (g) and parenchymal (h) mineralization is seen. (i,j) An extensive gliosis with strikingly high numbers of Rosenthal fibers is present in the parenchyma (some indicated by arrows) (i) and around vessels (arrows) (j). Staining: hematoxylin and eosin in a, c and e–j, Elastica van Gieson in b and d. Scale bars: 200 μm in a–c and f, 100 μm in d, e, g and j, 500 μm in h, and 50 μm in i.